Chemical reagentsDoxorubicin (Dox, Sigma Chemical Co., Steinheim, Germany) and Etoposide (WAKO Kliqqi
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Chemical reagentsDoxorubicin (Dox, Sigma Chemical Co., Steinheim, Germany) and Etoposide (WAKO, Osaka, Japan) were being utilized to induce apoptosis. Cells were developed to eighty confluence, treated with Dox in the indicated concentrations for twelve h, after which you can harvested. Puromycin (Invitrogen, Carlsbad, CA) was accustomed to block nonsense mediated mRNA decay (NMD) pathway [50]. C